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dnaA [2019-01-29 15:15:35]
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dnaA [2019-01-29 15:15:35]

AAA ATPase, replication initiation protein
Locus
BSU00010
Isoelectric point
6.03
Molecular weight
50.70 kDa
Protein length
446 aa Sequence Blast
Gene length
1341 bp Sequence Blast
Product
replication initiation protein
Essential
yes
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
410 1,750

Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase DnaC
  • Protein family

  • dnaA family (according to Swiss-Prot)
  • Domains

  • AAA + domain
  • Effectors of protein activity

  • SirA displaces DnaA from the replication origin PubMed
  • YabA inhibits co-operative binding of DnaA to the oriC DNA PubMed
  • DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either ParA, YabA or DnaN with the AAA domain of DnaA PubMed
  • interaction with DnaD inhibits the ability of DnaA to cooperatively bind to DNA PubMed
  • DNA gyrase (GyrA-GyrB) inhibits association of DnaA with oriC PubMed
  • Structure

  • 2Z4R (from Thermotoga maritima ) PubMed
  • Localization

  • throughout the cytoplasm PubMed
  • co-localizes with the origin region and replication forks throughout the cell cycle PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon
  • Regulation

  • negatively controlled by DnaA PubMed and Spo0A PubMed
  • Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of dnaA PubMed
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    Labs working on this gene/protein

  • Peter Graumann, Freiburg University, Germany homepage
  • Alan Grossman, MIT, Cambridge, MA, USA
  • Heath Murray, Centre for Bacterial Cell Biology, Newcastle, UK homepage
  • References

    Reviews

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    The DnaA regulon

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    Original publications

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